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    Thermostability of peroxidase in leaves of Buxus sempervirens L. under the influence of hyperthermic shock [Articol]
    (CEP USM, 2025) Cauș, Maria; Platcovschii, Nicolai
    The aim of this study was to investigate the thermal stability of total activity and peroxidase (PO) isoenzymes isolated from boxwood Buxus sempervirens (L.) leaves subjected to hyperthermic shock (HTS) at temperatures in the range of 53-90°C for 5 min. It was demonstrated that PO in leaves exposed to HTS of 53°C, 55°C and 57°C exhibits high resistance to these temperatures, the activity being at the level of the control. Although, the total activity and intensities of PO isoenzymes distributed in 4 zones in a vertical gradient (4-7%) of polyacrylamide gel (PAAG) gradually decreased with increasing HTS of temperatures above 58°C. However, treatment of leaves with HTS at 90°C for 5 min resulted in complete suppression of PO, the activity of which was not detected.