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Effect of drought on the total protein composition in sunflower seedlings [Articol]
(Editura USM, 2025) Rudacova, Angela; Cherdivara, Ala; Rudacov, Serghei
The aim of the work was to study the protein composition of seedlings of several sunflower genotypes under the influence of simulated drought. The protein composition was studied using SDS electrophoresis. The drought effect was created using PEG (6000). Drought-induced changes in protein composition were detected in only two of the six studied genotypes. In the Rf 2 genotype, under the influence of PEG (30%), 2 zones with low molecular weights accumulated over 6 days. In the ASC3 genotype, 3 zones of proteins with low molecular weights began to appear already at the first stage of drought, and they accumulated as the drought intensified. The amount of these zones increased under severe drought by almost 5-10 times compared to the control. The molecular weights of the proteins that accumulate during drought (D1, D2 and D3) range from 22 to 20 kDa, which is consistent with the molecular weights of dehydrins, which found in Helianthus annuus. The enhanced accumulation of these proteins under drought conditions may suggest that the ASC3 genotype exhibited greater drought tolerance and could be a promising parental line for breeding purposes.