The study of aquatic fungi is an area of major interest in microbiology, due to their essential ecological role and valuable biotechnological potential. In this context, the purpose of the paper was to test 71 strains of aquatic micromycetes isolated from the water basins of Chisinau municipality, in order to evaluate their antifungal activity against phytopathogens of agricultural importance. The results showed that over 80% of the analysed strains showed high antifungal activity, the most effective being against Fusarium solani (93%) and Botrytis cinerea (85.9%). The Trichoderma genus was shown to have remarkable antifungal potential, with inhibition areas ranging from 16 to 40 mm. Following the tests, two strains - T. atrobruneum CNMN FD 25 and T. longibrachiatum CNMN FD 27 - were selected to obtain biopreparations, which demonstrated increased efficiency in inhibiting the development of phytopathogenic fungi.
