2. Articole
Permanent URI for this collectionhttps://msuir.usm.md/handle/123456789/13419
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Item Transcriptome profiling of tomato response to phytoplasma infection(CEP USM, 2024) Mitina, Irina; Grajdieru, Cristina; Bahsiev, Aighiuni; Tumanova, Lidia; Zamorzaeva, Irina; Mitin, Valentin; Juříček, Miloslav; Muller, KarelTranscriptome sequencing is a valuable tool, which allows the researchers to study the dynamics of gene expression and identify genes, which control economically important traits. In this work, genes involved in tomato response to ‘Candidatus Phytoplasma solani’, a bacterial pathogen which can cause severe economic losses, were studied by transcriptome sequencing. As a result, 143 genes were found to be differentially expressed in the infected tomato plants, compared to healthy plants, 81 were upregulated and 62 downregulated. This analysis can shed light on the mechanisms of phytoplasma induced host gene expression regulation and on tomato response to phytoplasma infection.Item IMMUNOLOGIC EVALUATION OF MAIZE COLLECTION SAMPLES(CEP USM, 2023) Bilici, Elena; Grajdieru, CristinaSeven inbred maize lines from the active collection of Laboratory of plant genetic resources were tested for their susceptibility to fungal pathogens using visual evaluation of disease symptoms and molecular diagnostics. The highest infection rate was registered in MAN2492 and was 35%, therefore the respective genotype can be classified as susceptible. MAN2466 lacked symptoms of diseases and for MAN2488 minimum value of the respective parameter was registered (5%) – resistant genotype group. Quantitative analysis showed that the overall value of DNA sequences specific for the fungal genome was the lowest in the grain of MAN2488, MAN2491, MAN2470 genotypes.