METODĂ NOUĂ DE APRECIERE A TOXICITĂŢII SUBSTANŢELOR CHIMICE
| dc.contributor.author | Toderaș, Ion | |
| dc.contributor.author | Gulea, Aurelian | |
| dc.contributor.author | Gudumac, Valentin | |
| dc.contributor.author | Roșcov, Elena | |
| dc.contributor.author | Garbuz, Olga | |
| dc.date.accessioned | 2023-10-23T09:31:24Z | |
| dc.date.available | 2023-10-23T09:31:24Z | |
| dc.date.issued | 2018-09-21 | |
| dc.description.abstract | We developed a new invention that relates to method for assessing the toxicity of chemical substances.The method of the invention includes the preparation of the culture of Paramecium caudatum, adding to the samples investigated to test chemicals in various concentrations, incubating the sample to be studied and control the addition of colourant 3-amino-7-dimethylamino-2-methylphenazine hydrochloride, incubation with subsequent addition of solution of formalin, centrifuging the sample, removing the supernatant, adding the solution of hydroxide of sodium, determine the absorbance using a spectrophotometer, followed by calculating the percentage of paramecii viability and determine the lethal concentration (LC50) at the same time as the value of the concentration LC50 is small, the toxicity of the tested substance is higher. | en |
| dc.identifier.citation | TODERAŞ, Ion, GULEA, Aurelian, GUDUMAC, Valentin, ROȘCOV, Elena, GARBUZ, Olga. Metodă nouă de apreciere a toxicităţii substanţelor chimice. În: Functional Ecology of Animals: 70th anniversary from the birth of academician IonToderas:International symposium, 21 septembrie 2018, Chişinău: Imprimt Plus, 2018, pp. 454-463. ISBN 978-9975-3159-7-5 | en |
| dc.identifier.isbn | 978-9975-3159-7-5 | |
| dc.identifier.uri | https://msuir.usm.md/handle/123456789/11417 | |
| dc.identifier.uri | https://doi.org/10.53937/9789975315975.81 | |
| dc.language.iso | ro | en |
| dc.publisher | Imprimt Plus | en |
| dc.subject | Paramecium caudatum | en |
| dc.subject | chemical substances | en |
| dc.subject | colourant 3-ami- no-7-dimethylamino-2-methylphenazine hydrochloride | en |
| dc.subject | lethal concentration (LC50) | en |
| dc.subject | spectrophotometer | en |
| dc.title | METODĂ NOUĂ DE APRECIERE A TOXICITĂŢII SUBSTANŢELOR CHIMICE | en |
| dc.type | Article | en |