ОСОБЕННОСТИ ДЕЙСТВИЯ ЦИАНОБАКТЕРИЙ НА КОЛОРАДСКОГО ЖУКА В СВЕТЕ ГИСТОПАТОЛОГИЧЕСКОЙ ХАРАКТЕРИСТИКИ

Abstract

We have already discussed pathological effects of cyanobacteria on invertebrate organism in our papers. This study involved natural communities of Microcystis aeruginosa and Colorado potato beetle larvae of the second instar: food and insects taken separately and together were treated by 0.5% water suspension of cyanobacterial powder. The dynamics of inhibitory cyanobacterial action and histological alterations were observed in larvae during 7 days. The pathological process took place in midgut (epithelial hypersecretion, vacuolization, proliferation, degradation and desquamation), Malphigian tubes (caryolysis and desquamation in epithelium) and fat body (vacuolization and caryolysis) during the first 24 hours. During the following days these processes progressed (necrosis of midgut epithelium, muscle layer exposed and intestine wall destructed, total degradation and necrobiosis of fat body, dystrophy of Malphigian tubes), alterations in muscle fibres were observed (tissue homogenization, refining of fibres and dystrophy). Treatment of larvae caused the destruction of cuticle, hypoderm, respiratory system and subcuticular fat body. Complex treatment caused intense mortality.