ANALIZA GENETICO-MOLECULARĂ A UNOR SOIURI AUTOHTONE DE GLYCINE MAX (L.) MERRILL PRIN UTILIZAREA MARKERILOR MICROSATELITICI
Date
2014
Authors
Journal Title
Journal ISSN
Volume Title
Publisher
CEP USM
Abstract
Prin intermediul marcherilor Inter-simplu Secvenţe Repetitive (ISSR) a fost evaluată diversitatea genetică a speciei
Glycine max L. Analiza ISSR a soiurilor autohtone de soia a pus în evidenţă existenţa polimorfismului intraspecific cu
valoarea medie 25%. În spectrul variat de fragmente amplificate s-a remarcat prezenţa unor secvenţe specifice care pot
fi utilizate în calitate de marcheri moleculari în genotipare la soia. Profilul electroforetic al ampliconilor ISSR a permis
determinarea distanţei genetice şi construirea dendrogramei ce a reflectat similitudinea genetică la soiurile studiate.
Genotiparea germoplasmei de soia prezintă un instrument molecular util pentru realizarea cu succes a programelor
de selecţie moderne, scopul cărora este de a crea soiuri noi cu caractere dorite.
In this study, the genetic diversity of Glycine max L. was evaluated using inter-simple sequence repeat (ISSR) markers. Intraspecific polymorphism of soybean landraces was 25%. The molecular marking revealed the specific fragments, which can be used as molecular markers in so ybean genotyping. On the bases of ISSR analysis, the genetic distances were estimated, and dendrogram reflecting genetic similarity was constructed. Genotyping of soybean germplasm is useful tool for modern breeding programs which are aimed at the development of new cultivars with desirable traits.
In this study, the genetic diversity of Glycine max L. was evaluated using inter-simple sequence repeat (ISSR) markers. Intraspecific polymorphism of soybean landraces was 25%. The molecular marking revealed the specific fragments, which can be used as molecular markers in so ybean genotyping. On the bases of ISSR analysis, the genetic distances were estimated, and dendrogram reflecting genetic similarity was constructed. Genotyping of soybean germplasm is useful tool for modern breeding programs which are aimed at the development of new cultivars with desirable traits.
Description
Keywords
Glicine max, soiuri autohtone, polimorfism, fragmente specifice, distanţă genetică, markeri ISSR
Citation
BIVOL, Ina, BÎRSAN, Ana. Analiza genetico-moleculară a unor soiuri autohtone de Glycine max (L.) Merrill prin utilizarea markerilor microsatelitici. In: Studia Universitatis Moldaviae. Seria Ştiinţe reale şi ale naturii. Biologie. Chimie. Revistă ştiinţifică. 2014, nr 6.(76), pp. 50-56