Роль неорганических источников азотного питания для гифомицета Lecanicillium lecanii при культивировании в жидких средах глубинным методом [Articol]

Abstract

The nutrient medium was optimized by the nitrogen source to obtain an effective insecticidal biopreparation for plant protection based on the hyphomycete L. lecanii. The present study used a basic liquid molasses-sucrose nutrient medium with the addition of ammonium nitrate (NH4NO3) and sodium nitrate (NaNО3) in an amount of 3 g/L. No inorganic nitrogen source was added to control samples. The hyphomycete was cultured using the submerged method on a shaker at 200 rpm and 24°C for 96 hours. At the end of cultivation, the titer of the obtained suspensions was determined in a Goryaev chamber. Each sample was examined in the field of view of a microscope in a „crushed drop” preparation. As a result, the use of ammonium nitrate yielded a conidial suspension with a high titer of 1,0 × 10⁹ spores/mL, effective for protecting plants from sucking pests under greenhouse conditions. Nitrogen-containing components play a crucial role in enhancing the quality of a liquid biopreparation based on L. lecanii by promoting fungal growth, development, and mycelial maturation, ultimately leading to the production of a high-titer conidial suspension.