Facultatea de Biologie și Geoştiinţe / Faculty of Biology and Geosciences

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Author: search.filters.author.Port, AngelaEnd date: 2019

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    MOŞTENIREA GENELOR Rf LA DIVERSE GENOTIPURI DE FLOAREA-SOARELUI
    (CEP USM, 2010) Duca, Maria; Port, Angela; Midoni, Andrei; Anisimova, Irina; Rotaru, Tudor
    Research on four sources of pollen fertility restoration (I1-I3, F1, F2 şi BC1) revealed that this character was controlledin three cases (Drofa, Valentino and Sumbred 254) by one single dominant gene and in one case (Xenia) by two non-allelic dominant genes Rf
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    SOME PHYLOGENETIC ASPECTS OF DECORATIVE FISHES BASED ON MITOCHONDRIAL 12S AND 16S rDNA GENES
    (CEP USM, 2008) Duca, Maria; Port, Angela; Toderaş, Ion; Ciobanu, Lucia
    În lucrare sunt prezentate unele aspecte de analiză filogenetică a unor specii de peşti decorativi în baza procentului de similaritate a genelor mitocondriale 12S şi 16S rADN, fiind utilizată metoda PCR cu primeri specifici şi informaţia din baza de date NCBI, inclusiv programul CLUSTAL W. Analiza comparativă a pus în evidenţă cel mai înalt grad de omologie a genelor:12S–94% şi 16S–92% la Trichogaster trichopterus şi Trichogaster leerii din ordinul Perciformes. În cazul speciei Platydoras costatus, ordinul Silusiformes,s-au constatat cele mai mari divergenţe pentru ambele gene studiate.
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    ASPECTE PRIVIND ESTIMAREA GRADULUI DE STERILITATE A LINIILOR DE FLOAREA-SOARELUI
    (CEP USM, 2007) Duca, Maria; Port, Angela; Leviţchi, Alexei; Rotaru, Tudor
    Monitoring of the seed quality at different stages of plant breeding at sunflower represents a very important element in obtaining high yield hybrids based onCMS-Rfgenetic system. Sunflower represents a protandric plant with a single terminal inflorescence which contains 700-3000 individual hermaphrodite flowers, exposed on the calathidium, making manual emasculation almost impossible. That’s why the utilization of the male sterility was indispensable and necessary for the obtaining of the high level of hybridization at all the stages of sunflower breeding. Wide utilization of maternal lines, with cytoplasmic male sterility, and of paternal lines, with fertility restoring genes Rf, lead to the necessity to observe the stability and homogeneity of parental genotypes.Thus, for the paternal genotypes it is important to verify the presence and the stare of Rf genes, but maintaining male sterility is verified by the level of sterility control. Utilization of PCR-based methods with specific primers allows controlling genotype quality.
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    ASPECTE PRIVIND IMPACTUL POLUĂRII ASUPRA UNOR SPECII DE ARBORI DIN CINCI ZONE ALE mun. CHIŞINĂU
    (CEP USM, 2007) Duca, Maria; Port, Angela; Glijin, Aliona; Teleuţă, Alexandru; Casapu, Olga
    To date an unprecedented, rapid change in environmental conditionsis observed, which is likely to override the adaptive potential of plants, especially that of tree species with their long reproductive cycles. These environmental changes mainly originate from anthropogenic activities, which have caused air and soil pollution, acid precipitation, soil degradation, salinity, increasing UV-B radiation, climatechange, etc. Some of these stress factors may fluctuate signifi-cantly in intensity and duration on time scales of hours, days, seasons, or years;others may change slowly and gradually affect plant growth conditions. The aim of this review was to investigate the physiological response of three species of trees Acer platanoides L; Populus nigra and Aesculus hippocastanum L. from five sites zones with different pollution. There were analysed the following indices: dry weight and total water quantity, chlorophylls, carotenes and total proteins amount that gradually were affected. The significant variations of chlorophylls concentration demonstrate that the photosynthetic system is highly sensible to pollution factors. In conclusion, according to our assays the studied species of trees are stress tolerant to pollutants and can be used as bioindicators.
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    POLIMORFISMUL BIOCHIMIC LA PLANTELE GENULUI MENTHA
    (CEP USM, 2012) Ciobanu, Vasile; Pisov, Maria; Rezinciuc, Svetlana; Port, Angela; Duca, Maria; Reva, Veaceslav; Clapco, Steliana
    Electrophoretic protein profiles, oil composition and morphological traits were used to study the genetic diversity of wild-growing Mentha spicata and four hybrids from the collection of mint of the Moldova State University. It was found out that forms with different origin and morphological peculiarities are characterized by a similar essential oil composition, rich in the carvone 60-65% and menthol 58-70%. SDS-PAGE analysis revealed 30 polypeptide components with relative molecular weights in a range of 14,15-90,0 kDa, which showed molecular heterogeneity average of 43%. A negative correlation was found among morphological parameters (inflorescence, leaf and plant length) of Mentha spicata and quantity of the main terpenoid - carvone. Also, a more pronounced protein polymorphism was established in the wild-growing form.
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    POLIMORFISMUL RAPD-PCR LA PLANTELE GENULUI MENTHA
    (CEP USM, 2012) Duca, Maria; Port, Angela; Rezinciuc, Svetlana; Clapco, Steliana; Ciobanu, Vasile; Pisov, Maria
    In this study, the genetic diversity of one wild-growing form of Mentha spicata and four hybrids from the collection of mint of Moldova State University has been evaluated with random amplified polymorphic DNA (RAPD) markers. The highest level of molecular polymorphism was ascertained in wild-growing Mentha spicata. These results are important for the exploitation of new genetic materials of Mentha in plant amelioration and selection of genotypes with superior quality.
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    PLANTELE MEDICINALE DIN FLORA SPONTANĂ A REPUBLICII MOLDOVA ASPECTE MODERNE DE CERCETARE
    (Gradina Botanică (Institut) a AŞM, 2011) Duca, Maria; Port, Angela; Muller-Uri, Frieder; Leviţchi, Alexe; Budeanu, Oleg; Mârza, Mihai; Munteanu, Viorel; Ţapu, Lucia
    Medical plants represent an important resource of vegetal drugs and biological active compounds. Nowadays, it is recommended to integrate application of traditional medicine with modern one, especially regarding scientifi c research and assessment of natural drugs. Usage of local plants in medicine is a prospective and economically important direction. Flora of Republic of Moldova includes a vast diversity of species with opportunity to be applied in treatment of cardiovascular diseases, infections, oncology etc. But, for their exploitation it is necessary to apply modern technologies of research, which generates new knowledge for rational breeding of medical plants, extraction of pharmacologic substances and drug production. Articles present a synthesis of information on medicinal plants found in Moldova, biological aspects of their care. It evaluated the potential number of species and the posibility to store them in a database. One of the prospects for recovery of plant genetic fund may be provided by genotyping of medicinal plants by molecular biology techniques and bioinformatics analysis.