Facultatea de Biologie și Geoştiinţe / Faculty of Biology and Geosciences
Permanent URI for this communityhttps://msuir.usm.md/handle/123456789/4
Browse
6 results
Search Results
Item EFFICIENCY OF MICROSATELLITE MARKERS IN GENOTYPING OF OROBANCHE CUMANA POPULATIONS(Iasi University of Life Sciences, 2021-10-21) Mutu, Ana; Clapco, Steliana; Duca, MariaMicrosatellites or simple sequence repeats (SSRs) markers are widely dispersed across the genome, have a codominant and multiallelic nature, high variability and informativeness, and a specific chromosomal location. For this reason, they are playing a significant role in different fundamental and applicable fields, including genetic studies at the individual, population, species levels, and molecular breeding programs. In this study, the utility of 15 microsatellite markers in discrimination of 33 Orobanche cumana (broomrape) populations from different geographical locations was assessed: Republic of Moldova (11 populations), Romania (1), Bulgaria (4), Serbia (7), Turkey (7) and China (3). The PCR amplification products obtained showed differences by number and size depending on the markers used (3 and 16 alleles). A total of 110 alleles with fragment sizes ranged from 76-343 bp were determined. The evaluation of the genetic polymorphism of SSR markers was performed by the effective number of alleles per locus (Ne) with an average of 5.31, Polymorphic Information Content index (PIC) – 0.75, Nei's genetic diversity index (H) – 0.78 and, Resolving Power index (Rp) – 5.79. High values of all indices revealed the significant informative capacity and effectiveness of markers studied. At the same time, most markers showed PIC values higher than 0.5, indicating a high polymorphism in O. cumana populations. Seven microsatellite markers (Ocum-052, Ocum-059, Ocum-074, Ocum-081, Ocum-087, Ocum-196, Ocum-197) were selected based on the analyzed statistical parameters, suggesting that these markers can efficiently measure genetic diversity in broomrape. Based on the Rp index and PIC values of these 15 SSR markers, genetic diversity of Turkish (Rp: 4.774; PIC: 0,722) and Moldavian (Rp: 4.394; PIC: 0,716) Orobanche cumana populations was higher than in other populations. However, the studied microsatellite (SSR) markers system characterized very well the genetic structure of all O. cumana populations included in this study. Selected markers could eventually be useful for breeders and sunflower seed producers to improve their control strategies for this parasitic plant.Item EFICIENȚA UNOR MARCHERI MOLECULARI ÎN DISCRIMINAREA POPULAȚIILOR DE LUPOAIE ORIGINARE DIN CHINA(Tipografia „Print Caro”, 2021) Duca, Maria; Mutu, Ana; Bivol, Ina; Clapco, StelianaIn this study, the effectiveness of different types of molecular markers in assessing genetic diversity of populations of O. cumana from China was determined. ISSR and SSR markers detected different levels of genetic variability among and within broomrape populations. SSR markers analysis showed high level of genetic variation within the populations as revealed by high average values of Nei's gene diversity (H=0,75) and Shannon's information index (I=1,44), while genotyping with ISSR markers showed greater ability to discriminate genotypes according to Resolving power (Rp=7,24). Thus, the combined use of ISSR and SSR markers allowed the detection of higher polymorphism than either set of marker alone.Item VARIABILITATEA UNOR POPULAȚII DE LUPOAIE ORIGINARE DIN CHINA. MORFOMETRIA ȘI IDENTIFICAREA RASELOR (I)(Academia de Ştiinţe a Moldovei, 2021) Duca, Maria; Mutu, Ana; Clapco, Steliana; Bivol, Ina; Wang, ChaoThe article is a synthesis of some studies on the identification of broomrape races originating from China and the determination of the level of intra- and interpopulation variability based on the morphometric parameters of the seeds. The racial status of the analyzed populations was established, also two races of broomrape were identified: race G (Ch1 from Inner Mongolia and Ch3 from HeBei) and race H (Ch2 from Inner Mongolia) using internationally recognized differential Or homosygous lines set. Comparative analyses on the morphometric characteristics of broomrape revealed significant differences in seed Length (Ch1 and Ch3 populations, race G) and Length/width ratio (Ch3 and Ch4), which is an indication of the moderate level of interpopulation variability. In the case of assess of the intrapopulation variability level, the relatively homogeneous (Ch2, Ch3 and Ch4) and relatively heterogeneous (Ch1) populations were identified. According to the coefficient of variation, the population Ch2 identified as H broomrape race has distinguished as the most homogeneous for the investigated biometric indices of the seeds. The moderate positive correlations (between Length and width seed, between seed length and L/w ratio), the strong positive correlations (between Length and L/w ratio) and also the strong negative correlations (between width and L/w ratio) were established. The results obtained through Pearson coefficient revealed the discrimination of populations by region.Item STUDIES OF BROOMRAPE POPULATIONS ASSOCIATED WITH INCREASED GENETIC DIVERSITY(CEP USM, 2021) Bivol, Ina; Mutu, AnaIn the present investigation an effort was made to identify of broomrape races originating from China and study the level of intra- and interpopulation variability based on the morphometric parameters of the seeds and also the molecular markersItem VARIABILITATEA UNOR POPULAȚII DE LUPOAIE ORIGINARE DIN CHINA. MORFOMETRIA ȘI IDENTIFICAREA RASELOR (I)(Academia de Ştiinţe a Moldovei, 2021) Duca, Maria; Mutu, Ana; Clapco, Stetlana; Wang, ChaoThe article is a synthesis of some studies on the identification of broomrape races originating from China and the determination of the level of intra- and interpopulation variability based on the morphometric parameters of the seeds. The racial status of the analyzed populations was established, also two races of broomrape were identified: race G (Ch1 from Inner Mongolia and Ch3 from HeBei) and race H (Ch2 from Inner Mongolia) using internationally recognized differential Or homosygous lines set. Comparative analyses on the morphometric characteristics of broomrape revealed significant differences in seed Length (Ch1 and Ch3 populations, race G) and Length/width ratio (Ch3 and Ch4), which is an indication of the moderate level of interpopulation variability. In the case of assess of the intrapopulation variability level, the relatively homogeneous (Ch2, Ch3 and Ch4) and relatively heterogeneous (Ch1) populations were identified. According to the coefficient of variation, the population Ch2 identified as H broomrape race has distinguished as the most homogeneous for the investigated biometric indices of the seeds. The moderate positive correlations (between Length and width seed, between seed length and L/w ratio), the strong positive correlations (between Length and L/w ratio) and also the strong negative correlations (between width and L/w ratio) were established. The results obtained through Pearson coefficient revealed the discrimination of populations by region.Item STUDIES OF BROOMRAPE POPULATIONS ASSOCIATED WITH INCREASED GENETIC DIVERSITY(CEP USM, 2021) Bivol, Ina; Mutu, AnaIn the present investigation an effort was made to identify of broomrape races originating from China and study the level of intra- and interpopulation variability based on the morphometric parameters of the seeds and also the molecular markers. The racial status of the analyzed populations was established, also two races of broomrape were identified: race G (Ch1 population from Inner Mongolia and Ch3 from Hebei) and race H (Ch2 from Inner Mongolia) using internationally recognized a set of differential Or homozygotes lines. Comparative analyzes on the morphometric characteristics of broomrape seeds performed by descriptive statistical analysis and correlative association revealed significant differences in seed length (Ch1 and Ch3) and length/width ratio (Ch3 and Ch4 from Xianjiang), which is an indication of the moderate level of interpopulation variability. In the case of assess of the intrapopulation variability level, depending on the coefficient of variation of the analyzed parameter, the relatively homogeneous (Ch2, Ch3 and Ch4) and relatively heterogeneous (Ch1) populations were identified. Genetic variability between and within some broomrape populations from different regions of China was also explored using 15 SSR and 14 ISSR markers. SSR and ISSR markers utility was evaluated by calculating the polymorphism information content, observed and effective number of alleles, Nei's and Shannon diversity index, number and percentage of polymorphic loci. The high intrapopulation genetic variability was revealed by SSR markers while as for the ISSR primers a high degree of genetic variations was found at the interpopulation level. UPGMA clustering and principal component analysis performed according to ISSR and SSR/ISSR data set allowed to clearly distinguished all the broomrape populations for geographical origin and their racial origin while, in the case of SSR markers did not find this type of correlation. In conclusion, although the morphological markers were not found better than molecular, after all a combination of both markers would be highly efficient in detecting genetic variability and phylogenetic analysis among different populations of Orobanche cumana.