Facultatea de Biologie și Geoştiinţe / Faculty of Biology and Geosciences

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Author: search.filters.author.Clapco, StelianaSubject: search.filters.subject.broomrape

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    Factori de transcripție implicați în răspunsul florii-soarelui la acțiunea lupoiaei [Articol]
    (2022) Duca, Maria; Martea, Rodica; Port, Angela; Clapco, Steliana
    The present research is focused on bioinformatics and genes expression analysis aiming to elucidate new aspects of resistance mechanisms in the experimental model of the sunflower-broomrape interaction. The qPCR analysis of WHY1, TGA2 and TGA5 transcription factors revealed transient, oscillating changes in the accumulation of mRNA in plants cultivated in the absence and presence of pathogen in dynamics. The highest level of relative expression of WHY1 factor was attested in the roots of host plant during the stage of aerial shoots development (67 days), contrary to factors belonging to TGA family, which showed minimal values of the relative expression at the same stage of development. In the most of cases WHY1, TGA2 and TGA5 genes were found to be suppressed in the roots of sunflower-resistant genotypes under biotic stress. The higher number of gene interactions is due to co-expression in the case of WHY1 (93.68 %) and the presence of common protein domains in the case of TGA family factors (70 %). Associations between the NPR1 gene with TGA2 (46 connections) and TGA5 (33 interactions) have also been identified. The highest fluctuations in the level of relative expression of the NPR1 gene (key element in the manifestation of plant resistance) are found in the sensitive genotype Performer, in the case of non infested plants and in resistant hybrid PR64LE20 under biotic stress.
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    EFICIENȚA UNOR MARCHERI MOLECULARI ÎN DISCRIMINAREA POPULAȚIILOR DE LUPOAIE ORIGINARE DIN CHINA
    (Tipografia „Print Caro”, 2021) Duca, Maria; Mutu, Ana; Bivol, Ina; Clapco, Steliana
    In this study, the effectiveness of different types of molecular markers in assessing genetic diversity of populations of O. cumana from China was determined. ISSR and SSR markers detected different levels of genetic variability among and within broomrape populations. SSR markers analysis showed high level of genetic variation within the populations as revealed by high average values of Nei's gene diversity (H=0,75) and Shannon's information index (I=1,44), while genotyping with ISSR markers showed greater ability to discriminate genotypes according to Resolving power (Rp=7,24). Thus, the combined use of ISSR and SSR markers allowed the detection of higher polymorphism than either set of marker alone.
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    FACTORI DE MEDIU ASOCIAȚI CU INCIDENŢA PATOGENILOR LA CULTURA DE FLOAREA-SOARELUI
    (CEP USM, 2021) Duca, Maria; Clapco, Steliana; Burcovschi, Ion; Tabacari, Ruslan; Domenco, Rodion
    Prezentul studiu s-a axat pe analiza influenței unor factori de mediu (temperatura și cantitatea de precipitații) asupra incidenței patogenilor florii-soarelui în diferite zone ale Republicii Moldova. Au fost analizate date privind infecția naturală a unui set de 7-15 hibrizi de floarea-soarelui cultivați în diferite zone pedoclimatice (Nord – Visoca, Soroca; Pelinia, Drochia; Centru – Băcioi, Chișinău; Sud – Grigorievca, Căușeni; Svetlâi, Comrat), în perioada 2015-2020, fiind relevată prevalența tulpinilor fungice în partea de nord și în cea de centru ale țării, caracterizate prin cele mai mari cantități de precipitații, și a angiospermei lupoaia – preferențial în partea de sud, cu indici de temperatură mai înalți.