The researches results for vitamin complex impact on morphometric indicators on potato plants in culture in vitro proposed in the paper. The vitamin complex in concentration 2,0 and 2,5 ml/l add to Murashige-Skoog medium was the most effective for test tube plant morphogenesis cultivar Chervona ruta. There were determined the internodes increase; roots quantity, plants height, plants length respectfully:5,6 and 5,9 pcs, 7,0 and 7,3 pcs, 80,2 and 92,8 mm, 82,7 and 89,6 mm.