Institutul de Genetică, Fiziologie şi Protecţie a Plantelor

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Subject: search.filters.subject.antifungal activity

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    Aquatic basins – natural source of fungi with antifungal potential [Articol]
    (Editura USM, 2025) Sîrbu, Tamara; Moldovan, Cristina
    The study of aquatic fungi is an area of major interest in microbiology, due to their essential ecological role and valuable biotechnological potential. In this context, the purpose of the paper was to test 71 strains of aquatic micromycetes isolated from the water basins of Chisinau municipality, in order to evaluate their antifungal activity against phytopathogens of agricultural importance. The results showed that over 80% of the analysed strains showed high antifungal activity, the most effective being against Fusarium solani (93%) and Botrytis cinerea (85.9%). The Trichoderma genus was shown to have remarkable antifungal potential, with inhibition areas ranging from 16 to 40 mm. Following the tests, two strains - T. atrobruneum CNMN FD 25 and T. longibrachiatum CNMN FD 27 - were selected to obtain biopreparations, which demonstrated increased efficiency in inhibiting the development of phytopathogenic fungi.
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    Antifungal activity of lipid fractions of medicinal plants against Furasium isolates from infected garlic cloves [Articol]
    (Editura USM, 2025) Bobrova, Olena; Golosna, Lesia; Narozhnyi, Stanislav; Osetsky, Aleksandr; Zamecnik, Jiri
    Garlic (Allium sativum L.) is widely cultivated for its culinary and medicinal value but is highly susceptible to fungal diseases, particularly those caused by Fusarium species. This study evaluated the antifungal activity of lipid fractions from seven medicinal plants against Fusarium isolates obtained from infected garlic cloves. The lipid fractions were added to PDA medium and fungal growth was monitored over nine days. The lipid fractions of Eucalyptus mellidora and Salvia officinalis significantly reduced mycelial growth of both isolates compared to the control, suggesting strong antifungal potential. Other oils exhibited moderate or low activity. These findings support the potential use of selected plant-derived lipid fractions as natural fungicides in garlic protection. Further research is needed to validate efficacy under field conditions.
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    Antagonistic activity of bacterial isolates against fusarium pathogens
    (CEP USM, 2024) Bîrsa, Maxim; Balan, Ludmila; Bogdan-Golubi, Nina; Moldovan, Cristina
    Actinobacteria and bacteria stored in NCNM were tested against F. oxysporum CNMN-FF-06 and F. solani CNMN-FF-07. In case of antagonistic activity against F. solani, results were much higher in comparison with F. oxysporum, ranged between 12.3 – 19.3 mm. The highest result of antifungal activity of bacteria against F. oxysporum was registered for the strain Bacillus velezensis CNMN-BB-16 (25.0 mm). For F. solani, the highest result was registered for the strain Micrococcus yunnanensis CNMN-BM-19 (33.0 mm). Results of the present research had shown that biocontrol agents can be used as an alternative for chemical pesticides utilized against phytopathogenic fungi.
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    Acțiunea antifungică a trichodermei atrobrunneum asupra unor fungi fitopatogeni cultivată în prezența unor substanțe utilizate în calitate de stimulatori în mediul de cultivare
    (CEP USM, 2024) Țurcan, Olga; Sîrbu, Tamara; Moldovan, Cristina; Chiselița, Oleg; Chiselița, Natalia
    The action of the cultivation medium and some substances used as stimulators on the productivity of the T. atrobrunneum CNMN FD 25 strain was studied. The obtained results allow us to summarize that of all the variations of media used for the cultivation of T. atrobrunneum, the M(Gl,Z) medium proved to be the most optimal, registering a productivity of 15 g/l. T. atrobrunneum cultivated on nutrient medium M (Gl, Z) has a strong antagonistic effect against phytopathogenic fungi: Alternaria alternata, Botrytis cinerea, Fusarium solani, Fusarium oxysporum, the diameter of the inhibition zones varied from 20 to 32 mm in relation to strain grown on M (Gl) and M (Z) medium, supplemented with iron compounds and exometabolites of S. cerevisiae.
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    THE USE OF TRICHODERMA SPECIES AGAINST THE MAIN MYCOTIC PATHOGENS OF WALNUT
    (CEP USM, 2023) Crucean, Ștefan; Șcerbacova, Tatiana; Voloșciuc, Leonid
    Walnut plantations suffer from the impact caused by a number of pathogenic mycotic microorganisms. The most common pathogen fungus is Alternaria alternata and Fusarium poae. In the framework of the research, we aimed to determine the antagonistic activity of the strain Trichoderma harzianum CNMN - FD - 16 against the identified pathogens. Thus, on the 10th day the inhibition of the pathogen A. alternata by T. harzianum is 74%. On the same day, the inhibition of F. poae by T. harzianum is 73%. Trichoderma harzianum suppressed the colonies of A. alternata of 83% - this means 4 points, and the suppression of the colonies of F. poae was 69% - registering 3 points.