In this work we aimed eestablishing the factors responsible for acid-base balance and buffering capacity assessment of natural waters. For rivers, buffering capacity values are: r. Dunăre (0,1-1,46 mmolechiv/l); r. Dniester (0,08-1,5 mmolechiv./l); for lakes – La Izvor (0,07-1,3 mmolechiv/l) and Valea Trandafirilor (0,11-1,27 mmolechiv/l). Following measurements made in natural waters, I noticed that operates a complex buffer system, consisting of biotic and abiotic.