Browsing by Author "Wilson, Karl"

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    IN VIVO AND IN VITRO LIMITED PROTEOLYSIS OF PHASEOLIN: FACTS, SUGGESTIONS AND PROBLEMS
    (CEP USM, 2007) Rudacova, Angela; Rudacov, Serghei; Lapteva, Natalia; Morari, Diana; Stepurina, Tatiana; Rotari, Vitalie; Kakhovskaya, Irina; Wilson, Karl; Fukuda, Takako; Utsumi, Shigeru; Shutov, Andrei
    Degradarea proteolitică a fazeolinei - globulinei 7S de rezervă din seminţele Phaseolus vulgarisare loc in vivo în timpul germinării şi este catalizată de acţiunea succesivă a legumainei Asp-specifice LP şi a enzimei de tip papainic PP. A fost studiată componenţa fragmentelor cu masă moleculară naltă care se formează la proteoliza limitată a fazeolinei la acţiunea LP şi la acţiunea succesivă a PL şi a PP in vivo şi, de asemenea, în cotiledoanele plantei în creştere.
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    LIMITED PROTEOLYSIS OF OAT 11S GLOBULIN BY PAPAIN
    (CEP USM, 2014) Shutov, Andrei; Rudakova, Angela; Klimova, Natalia; Lapteva, Natalia; Makaeva, Ellina; Wilson, Karl
    Limited proteolysis was found to occur during papain proteolysis of the oat 11S globulin. The initial limited proteolysis is extremely quick and consists of an N-terminal and C-terminal truncation of α-chains and C-terminal truncation of β-chains. Further on, the α-chain remnants are cleaved into similarly sized N-terminal and C-terminal fragments. Similar pattern of the globulin limited proteolysis was observed during germination of the oat seeds. Functional role of limited proteolysis in degradation of seed 11S globulins is discussed