Browsing by Author "Tagadiuc, Olga"

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Coordination compound as inhibitor of superoxid radical [Articol]
(2019) Garbuz, Olga; Pantea, Valeria; Usataia, Irina; Graur, Vasilii; Țapcov, Victor; Sardari, Veronica; Tagadiuc, Olga; Gudumac, Valentin; Gulea, Aurelian
A fost obținut un nou compus coordinativ biologic activ, nitratul de cupru [2 - ({2 [(ethylsulfanyl) (prop-2-en-1-yl) carbononoimidooyl] -hydrazinylidene} methyl) phenolate], care face parte din clasa isotiosemicarbazidelor metalelor de tranziție.S-a stabilit că acesta exercită proprietăți anti radicalice performante la acțiunea moleculei organice cu radicalul superoxidic. Datorită acestei poroprietăți compusul obținut poate avea o potențială aplicare in medicină in calitate de inhibitor al radicalilor superoxidici in corpul uman, care poate duce la prevenirea deteriorării tesutului cellular, aterosclerozii si carcinogenezei.Compusul coordinativ sintetizat extinde arsenalul inhibitorilor de radicali superoxidici cu activitate biologică importanta.
Influence of copper coordination compounds and cyanobacterian remedy BioR on bone collagen and hydroxyproline concentrations (ontogenetic view) [Articol]
(Elan-Poligraf, 2011-07-07) Tagadiuc, Olga; Gulea, Aurelian; Rudic, Valeriu; Gudumac, Valentin
The aim of our research was to assess the influence of copper coordination compounds CMT-28 and CMT-67 and cyanobacterian remedy BioR on bone collagen and hydroxyproline (HYP) concentrations at different stages of postnatal ontogenesis in normal physiological conditions and experimental osteopathy (EO). The survey was conducted on a sample of 301 white laboratory rats of both sexes, which were divided depending on their stage of postnatal ontogenetic development and experimental model: control; animal with experimental osteopathy (EO); animals with EO+CMT-28 1 mg/kg body weight; animals with EO+CMT-67 1 mg/kg body weight; animals with EO+BioR 1mg/kg body weight; animals with EO+BioR 2 mg/kg body weight; animals with EO+CMT-28+BioR (1 mg/kg body weight each); animals with EO+BioR+CMT-67 (1 mg/kg body weight each). In physiological conditions the ontogenetic modifications of the bone collagen and HYP content are statistically conclusive and of the same orientation, the maximum concentration was identified in adult animals and the minimum at advanced stages of postnatal ontogenesis regardless of sex. Experimental osteopathy is characterized by age and sex dependent changes of the collagen and hydroxyproline concentrations in bone, the most significant changes being revealed in young females. Copper coordination compounds, BioR and their combinations increase the turnover of the collagen in bone in EO that is accompanied by decrease of collagen and increase of the HYP concentration that is probably a compensatory mechanism oriented to the replacement of the damaged collagen and the restoration of its normal nanostructure with the recovery of the bone.
Modificările ontogenetice ale nivelului de sulfaţi în ţesutul osos în condiţii fiziologice, osteopatia exprimentală şi la administrarea unor remedii [Articol]
(2011) Tagadiuc, Olga; Rudic, Valeriu; Gulea, Aurelian
Modificările sistemului glutationic eritrocitar în normă şi astm bronşic asociat sub acţiunea unor compuşi biologici activi autohtoni [Articol]
(2016) Pantea, Valeriana; Tagadiuc, Olga; Procopișin, Larisa; Andronache, Lilia; Gulea, Aurelian; Gudumac, Valentin
S-a studiat infl uenţa compuşilor biologic activi (CBA) autohtoni - CMD-4, CMD-8, CMJ-33 şi CMJ-23 asupra indicilor sistemului glutationic eritrocitar (GSH, GR, GPO, GST, G-6-PDH) in vitro în condiţii normale şi în astmul bronşic (AB) de severitate diferită. S-a stabilit că CBA autohtoni exercită efecte modulatoare relevante asupra pricipalelor enzime ale metabolismului GSH, preponderent stimulatoare, fapt ce ar putea fi apreciat ca un răspuns pozitiv ce poate proteja celulele de efectele toxice ale stresului oxidativ specifi c pentru AB. Indicii studiați pot fi folosiți ca biomarcheri informativi pentru monitorizarea procesului patologic și aprecierea efi cienţei preparatelor noi la restabilirea biochimică şi funcţională a sistemelor de protecție celulare în AB și diverse leziuni pulmonare.
New inhibitors of glycation and of the formation of advanced glycation end products [Articol]
(Academia de Ştiinţe a Moldovei, 2015) Andronache, Lilia; Gudumac, Valentin; Gulea, Aurelian; Tagadiuc, Olga; Garbuz, Olga; Pantea, Valeriana
Thiol-disulfide metabolism in kidney tissue at the administration of some copper coordination compounds [Articol]
(2020) Sardari, Veronica; Pantea, Valeriana; Gulea, Aurelian; Tagadiuc, Olga; Andronache, Lilia; Șveț, Inna; Țapcov, Victor; Gudumac, Valentin
Thiol-disulfide metabolism is essential for normal function of the organism. Thus the interest of the scientists in this area of research continues to grow. Material and methods: Copper coordination compounds (CCC), derivatives of thiosemicarbaside (CMD-4, CMJ-33, CMT-67), action on thiol-disulfide metabolism in the healthy Ratta albicans kidneys were studied. The animals were divided in 6 groups of 7 rats each. The control group included healthy rats which were injected i/m physiological solution 3 times a week, for 30 days. The rats from groups 2-6 have got 3 times a week, for 30 days, i/m injections of CCC. The activity of following thiol-disulfide metabolism enzymes in the renal supernatant has been measured: glutathion-reductase (GR), glutathion-peroxidase (GPO), glutathion-S-transferase (G-S-T), γ-glutamyl transpeptidase (γ-GTP), glutaredoxin (Grx), as well the amount of the protein SH-groups and of the total glutathione, reduced glutathione (GSH) and oxidized glutathione (GSSG) in renal tissue. Results: The compounds exhibit different actions: CMT-67 in the dose of 0.1 μM/kg influenced the activity of the glutathione metabolism enzymes – activated γ-glutamyl transpeptidase (γ-GTP) and glutaredoxine (Grx) and inhibited glutathione reductase (GR), while CMD-4 in doses of 0.1 μM/kg and 1.0 μM/kg and CMJ-33 in the dose of 1.0 μM/kg significantly diminished the reduced glutathione (GSH) level and increased the amount of the oxidised one (GSSG). Conclusions: Selective action of the copper coordination copounds established by this study opens new possibilities of their usage in the therapy of kidney diseases.
Use of micromolecular copper complexes of thiosemicarbazides as an endogenous catalase inducer/activator [Articol]
(2024) Andronache, Lilia; Pantea, Valeriana; Gulea, Aurelian; Tagadiuc, Olga; Matcovschi, Valerii
Introduction. Oxidative stress is a major mechanism in the pathogenesis of many diseases,including severe multifactorial ones. Catalase (CAT), along with superoxide dismutase, is one of the first-line antioxidant defense enzymes. The development of methods/compounds that can increase/induce CAT will provide new possibilities to strengthen antioxidant protection and prevent oxidative damage to cells and tissues. Material and methods. The ability of three copper coordination compounds from the class of transition metal thiosemicarbazides to enhance/induce CAT in erythrocytes of healthy white laboratory rats has been studied. Results. Dichloro-(methyl-N-(prop-2n-1-yl)-2-(pyridin-2-ylmethylidene)hydrazine-carbimi do-thioate)copper was found to show the highest induction and/or activation of CAT, exceeding 2.71 times the values of the control group and 1.80 times the values produced by vitamin D3. This reveals increased synthesis of CAT after exposure to this compound, a phenomenon that we have established for the first time. Conclusions. The compound can be used as a therapeutic agent, which, by activating the production of CAT in the body, can prevent and/or reduce the occurrence of multifactorial diseases, due to prevention of the damage of cells associated with excessive accumulation of hydrogen peroxide. The obtained data open perspectives for the research of biologically active synthetic compounds, which will diversify the arsenal of effective tools for preventing/treating various diseases.