Browsing by Author "Matcovschi, Valerii"

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Micromolecular inhibitors of superoxide radicals [Articol]
(2021) Andronache, Lilia; Pantea, Valeriana; Gulea, Aurelian; Șveț, Inna; Graur, Vasilii; Matcovschi, Valerii; Gamaniuc, Marina; Gudumac, Valentin
Background: Currently, there is a growing interest in new copper (Cu2+) heterocyclic coordination compounds (CC), isothiosemicarbazide derivates, which demonstrated multiple beneficial properties, but their effect on reactions with free radicals such as the superoxide radical has not been investigated. Material and methods: The action of new micromolecular complexes of copper (Cu2+) chloride and bromide with methyl n- (prop-2-en-1-yl) -2- (pyridin2-ylmethylidene) hydrazine carbimidothioate on capturing activity of the superoxide radical was determined by the spectrophotometric method in vitro experiments. Results: It was established that the micromolecular complexes of copper (II) chloride and bromide with methyl n-(prop-2-en-1-yl)-2-(pyridin-2ylmethylidene) hydrazine carbimidothioate have been found to possess strong superoxide radical inhibitor properties when interacting with a superoxide radical. In addition to this, the IC50 of the studied compounds depends on the nature of the acid-ligand in the internal sphere of the complex and increases in the following sequence: Cl-–Br-. Conclusions: The established property of mentioned compounds is new, because their use as micromolecular inhibitors of superoxide radicals has not been described so far. The synthesized CC expand the arsenal of superoxide radical inhibitors with high biological activity. Their possible significance for the development of new treatment strategies for diseases associated with the overproduction of superoxide radicals is discussed.
Use of micromolecular copper complexes of thiosemicarbazides as an endogenous catalase inducer/activator [Articol]
(2024) Andronache, Lilia; Pantea, Valeriana; Gulea, Aurelian; Tagadiuc, Olga; Matcovschi, Valerii
Introduction. Oxidative stress is a major mechanism in the pathogenesis of many diseases,including severe multifactorial ones. Catalase (CAT), along with superoxide dismutase, is one of the first-line antioxidant defense enzymes. The development of methods/compounds that can increase/induce CAT will provide new possibilities to strengthen antioxidant protection and prevent oxidative damage to cells and tissues. Material and methods. The ability of three copper coordination compounds from the class of transition metal thiosemicarbazides to enhance/induce CAT in erythrocytes of healthy white laboratory rats has been studied. Results. Dichloro-(methyl-N-(prop-2n-1-yl)-2-(pyridin-2-ylmethylidene)hydrazine-carbimi do-thioate)copper was found to show the highest induction and/or activation of CAT, exceeding 2.71 times the values of the control group and 1.80 times the values produced by vitamin D3. This reveals increased synthesis of CAT after exposure to this compound, a phenomenon that we have established for the first time. Conclusions. The compound can be used as a therapeutic agent, which, by activating the production of CAT in the body, can prevent and/or reduce the occurrence of multifactorial diseases, due to prevention of the damage of cells associated with excessive accumulation of hydrogen peroxide. The obtained data open perspectives for the research of biologically active synthetic compounds, which will diversify the arsenal of effective tools for preventing/treating various diseases.