Browsing by Author "Bivol, Ina"

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ANALIZA GENETICO-MOLECULARĂ A UNOR SOIURI AUTOHTONE DE GLYCINE MAX (L.) MERRILL PRIN UTILIZAREA MARKERILOR MICROSATELITICI
(CEP USM, 2014) Bivol, Ina
Prin intermediul marcherilor Inter-simplu Secvenţe Repetitive (ISSR) a fost evaluată diversitatea genetică a speciei Glycine max L. Analiza ISSR a soiurilor autohtone de soia a pus în evidenţă existenţa polimorfismului intraspecific cu valoarea medie 25%. În spectrul variat de fragmente amplificate s-a remarcat prezenţa unor secvenţe specifice care pot fi utilizate în calitate de marcheri moleculari în genotipare la soia. Profilul electroforetic al ampliconilor ISSR a permis determinarea distanţei genetice şi construirea dendrogramei ce a reflectat similitudinea genetică la soiurile studiate. Genotiparea germoplasmei de soia prezintă un instrument molecular util pentru realizarea cu succes a programelor de selecţie moderne, scopul cărora este de a crea soiuri noi cu caractere dorite.
ANALYSIS OF MOLECULAR VARIANCE (AMOVA) OF OROBANCHE CUMANA POPULATIONS
(CEP USM, 2022-09-29) Duca, Maria; Mutu, Ana; Bivol, Ina
DISCRIMINATING ABILITY OF ISSR MARKERS IN THE REVEALING OF THE GENETIC POLYMORPHISM IN TURKISH BROOMRAPE POPULATIONS
(Trakya University Publisher, 2021-08-29) Duca, Maria; Bivol, Ina
In the present study, the analysis of the informativity level and discriminatory capacity for 14 ISSR-markers to identify polymorphism of seven Orobanche cumana populations from different geographical regions of Turkey was performed. ISSR markers utility was assessed by calculating of different parameters such as the polymorphism information content (PIC), observed and effective number of alleles (Na and Ne), Nei's and Shannon diversity indexes (H and I), number and percentage of polymorphic loci, resolving power (Rp), Simpson's coefficient (Hj). Out of 207 amplicons generated by microsatellites primers, 195 were found polymorphic (94.29%) and 12 were monomorphic with size range from 341 to 5353 bp. The average number of amplified bands was 14.79 fragments per primer. The PIC values for 14 primers ranged from 0.18 ((GATA)4) to 0.41 ((AG)8YA) with the mean value 0.34 Eight primers ((AG)8YA, (CT)8TC, BC807, BC841, (CA)6AC, (CTC)4RC, (CAG)5, BC835) were considered the most effective. The mean I value was 0.52 and ranged from 0.32 ((GATA)4) to 0.6 ((AG)8YA). The (AG)8YA, (CT)8TC, BC807, BC841, (CTC)4RC, (CA)6AC and (CAG)5 primers had the highest I values. The Rp ranged from 5.9 for primer (CA)6RG to 22.59 for primer (AG)8YA. Nine of the ISSR primers ((AG)8YA, (CTC)4RC, BC810, BC807, BC841, BC835, (CAG)5, BC857 and (CT)8TC) possessed high Rp values (22.59, 19.12, 16.83, 16.54, 16.49, 14.59, 13.32, 12.88 and 11.37, respectively) and were able to distinguish all 41genotypes. The higher discrimination potential calculating by the Simpson’s coefficient had primer BC810 (0.33) and the lower (CA)6RG (0.01), the mean value for all primers was 0.18. According to ISSR data analysis, the high proportion of the genetic variability (94.29%) was due to interpopulation variation and 45.82% of the diversity by cause of intrapopulation variations. In conclusion, in this comparative study of the discrimination capacity of 14 ISSR markers using to broomrape population genetic analysis, the tri- ((CTC)4RC, (CAG)5) and dinucleotide ((AG)8YA, BC807, BC841, (CT)8TC) primers proved more informative than tetranucleotide repeats. Moreover, these primers revealed the highest values for all analyzed polymorphism information indexes of genetic diversity (number of amplicons per primer from 15 to 28, level of polymorphism more than 88.89%, PIC≥0.36, I≥0.53, Hj≥0.11 and Rp≥11.37) for Turkish broomrape populations. The analysis of ISSR-markers showed that this type of marker is applicable for the assessment of intra- and interpopulation genetic polymorphism for species O. cumana.
EFICIENȚA UNOR MARCHERI MOLECULARI ÎN DISCRIMINAREA POPULAȚIILOR DE LUPOAIE ORIGINARE DIN CHINA
(Tipografia „Print Caro”, 2021) Duca, Maria; Mutu, Ana; Bivol, Ina; Clapco, Steliana
In this study, the effectiveness of different types of molecular markers in assessing genetic diversity of populations of O. cumana from China was determined. ISSR and SSR markers detected different levels of genetic variability among and within broomrape populations. SSR markers analysis showed high level of genetic variation within the populations as revealed by high average values of Nei's gene diversity (H=0,75) and Shannon's information index (I=1,44), while genotyping with ISSR markers showed greater ability to discriminate genotypes according to Resolving power (Rp=7,24). Thus, the combined use of ISSR and SSR markers allowed the detection of higher polymorphism than either set of marker alone.
EVALUATION OF POLYMORPHISM INFORMATION OF GENETIC DIVERSITY IN BROOMRAPE FROM BULGARIA
(CEP USM, 2022-09-29) Duca, Maria; Bivol, Ina
Finding an informative multilocus polymorphism in sunflower broomrape populations
(CEP USM, 2024) Bivol, Ina
Multilocus polymorphic DNA analysis was performed using 13 ISSR markers for 14 broomrape populations of Serbian and Moldovan origin. 279 ISSR fragments were identified for the whole sample, of which 265 (94.98%) were polymorphic. The seven groups of broomrape studied (whole sample, by country separately - Serbia and Moldova, and by race depending on the country - races ≤E serb, ≤E mold, H mold, H+ mold) showed different levels of genetic diversity. Based on these studies’ results, broomrape’s multilocus polymorphism was highly correlated with geographical origin, racial classification, and genetic background.
STUDIES OF BROOMRAPE POPULATIONS ASSOCIATED WITH INCREASED GENETIC DIVERSITY
(CEP USM, 2021) Bivol, Ina; Mutu, Ana
In the present investigation an effort was made to identify of broomrape races originating from China and study the level of intra- and interpopulation variability based on the morphometric parameters of the seeds and also the molecular markers. The racial status of the analyzed populations was established, also two races of broomrape were identified: race G (Ch1 population from Inner Mongolia and Ch3 from Hebei) and race H (Ch2 from Inner Mongolia) using internationally recognized a set of differential Or homozygotes lines. Comparative analyzes on the morphometric characteristics of broomrape seeds performed by descriptive statistical analysis and correlative association revealed significant differences in seed length (Ch1 and Ch3) and length/width ratio (Ch3 and Ch4 from Xianjiang), which is an indication of the moderate level of interpopulation variability. In the case of assess of the intrapopulation variability level, depending on the coefficient of variation of the analyzed parameter, the relatively homogeneous (Ch2, Ch3 and Ch4) and relatively heterogeneous (Ch1) populations were identified. Genetic variability between and within some broomrape populations from different regions of China was also explored using 15 SSR and 14 ISSR markers. SSR and ISSR markers utility was evaluated by calculating the polymorphism information content, observed and effective number of alleles, Nei's and Shannon diversity index, number and percentage of polymorphic loci. The high intrapopulation genetic variability was revealed by SSR markers while as for the ISSR primers a high degree of genetic variations was found at the interpopulation level. UPGMA clustering and principal component analysis performed according to ISSR and SSR/ISSR data set allowed to clearly distinguished all the broomrape populations for geographical origin and their racial origin while, in the case of SSR markers did not find this type of correlation. In conclusion, although the morphological markers were not found better than molecular, after all a combination of both markers would be highly efficient in detecting genetic variability and phylogenetic analysis among different populations of Orobanche cumana.
STUDIES OF BROOMRAPE POPULATIONS ASSOCIATED WITH INCREASED GENETIC DIVERSITY
(CEP USM, 2021) Bivol, Ina; Mutu, Ana
In the present investigation an effort was made to identify of broomrape races originating from China and study the level of intra- and interpopulation variability based on the morphometric parameters of the seeds and also the molecular markers
UNELE ASPECTE ALE STUDIULUI POTENŢIALULUI GENETIC LA HIBRIZI DE FLOAREA SOARELUI ÎN CONDIȚIILE REPUBLICII MOLDOVA
(CEP USM, 2023) Bivol, Ina; Burcovschii, Ion; Duca, Maria; Machidon, Mihail
În condiţiile zonei de nord și sud a Republicii Moldova pe parcursul anilor 2020-2021 s-a efectuat evaluarea unor hibrizi comerciali de floarea-soarelui cu diferită proveniență în scopul selectării hibrizilor cu caractere valoroase ca surse suplimentare pentru intensificarea producției de floarea-soarelui în țară. S-a stabilit că hibrizii testaţi au prezentat după trăsăturile morfologice o variabilitate bogată, care în mare măsură este dependentă de genotip, proveniența acestuia și totodată condițiile climatice zonale și anuale. Lucrarea de față include date privind evaluarea potenţialului genetic la 6 hibrizi de floarea-soarelui după unele caractere cantitative (indicele suprafeței foliare, suprafața foliară, cantitatea de clorofila a și b, cantitatea de carotenoizi, înălţimea plantei, numărul de frunze, recolta medie, conținutul de ulei) şi markeri moleculari (RAPD).
VARIABILITATEA UNOR POPULAȚII DE LUPOAIE ORIGINARE DIN CHINA. MORFOMETRIA ȘI IDENTIFICAREA RASELOR (I)
(Academia de Ştiinţe a Moldovei, 2021) Duca, Maria; Mutu, Ana; Clapco, Steliana; Bivol, Ina; Wang, Chao
The article is a synthesis of some studies on the identification of broomrape races originating from China and the determination of the level of intra- and interpopulation variability based on the morphometric parameters of the seeds. The racial status of the analyzed populations was established, also two races of broomrape were identified: race G (Ch1 from Inner Mongolia and Ch3 from HeBei) and race H (Ch2 from Inner Mongolia) using internationally recognized differential Or homosygous lines set. Comparative analyses on the morphometric characteristics of broomrape revealed significant differences in seed Length (Ch1 and Ch3 populations, race G) and Length/width ratio (Ch3 and Ch4), which is an indication of the moderate level of interpopulation variability. In the case of assess of the intrapopulation variability level, the relatively homogeneous (Ch2, Ch3 and Ch4) and relatively heterogeneous (Ch1) populations were identified. According to the coefficient of variation, the population Ch2 identified as H broomrape race has distinguished as the most homogeneous for the investigated biometric indices of the seeds. The moderate positive correlations (between Length and width seed, between seed length and L/w ratio), the strong positive correlations (between Length and L/w ratio) and also the strong negative correlations (between width and L/w ratio) were established. The results obtained through Pearson coefficient revealed the discrimination of populations by region.