Browsing by Author "Rotaru, Tudor"

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    ASPECTE PRIVIND ESTIMAREA GRADULUI DE STERILITATE A LINIILOR DE FLOAREA-SOARELUI
    (CEP USM, 2007) Duca, Maria; Port, Angela; Leviţchi, Alexei; Rotaru, Tudor
    Monitoring of the seed quality at different stages of plant breeding at sunflower represents a very important element in obtaining high yield hybrids based onCMS-Rfgenetic system. Sunflower represents a protandric plant with a single terminal inflorescence which contains 700-3000 individual hermaphrodite flowers, exposed on the calathidium, making manual emasculation almost impossible. That’s why the utilization of the male sterility was indispensable and necessary for the obtaining of the high level of hybridization at all the stages of sunflower breeding. Wide utilization of maternal lines, with cytoplasmic male sterility, and of paternal lines, with fertility restoring genes Rf, lead to the necessity to observe the stability and homogeneity of parental genotypes.Thus, for the paternal genotypes it is important to verify the presence and the stare of Rf genes, but maintaining male sterility is verified by the level of sterility control. Utilization of PCR-based methods with specific primers allows controlling genotype quality.
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    MOŞTENIREA GENELOR Rf LA DIVERSE GENOTIPURI DE FLOAREA-SOARELUI
    (CEP USM, 2010) Duca, Maria; Port, Angela; Midoni, Andrei; Anisimova, Irina; Rotaru, Tudor
    Research on four sources of pollen fertility restoration (I1-I3, F1, F2 şi BC1) revealed that this character was controlledin three cases (Drofa, Valentino and Sumbred 254) by one single dominant gene and in one case (Xenia) by two non-allelic dominant genes Rf
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    POSIBILITĂŢI DE ESTIMARE A PURITĂŢII GENETICE LA FLOAREA-SOARELUI
    (CEP USM, 2007) Duca, Maria; Leviţchi, Alexei; Rotaru, Tudor
    The efficiency of application of different techniques to monitor the biologic purity of initial material designed for the amelioration ensures the success of breeding programs. This means allow to estimate both, the homogeneity of inbred lines and the polymorphism of in frame of populations. Field tests are much easier and exact, but are time consuming, whereas laboratory tests provide fast results obtaining and reduce the expences for plant breeding programs. The utilization of different existant methods are determined by the budget, developed by the breeders, for the analyses and by the level of technical equipping of the laboratories. The most used method is based on the utilisation of storage protein electrophoretic spectra, being relative cheap and sufficiently exact.