Browsing by Author "Muller, Karel"

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    Molecular diagnostics of some fungal pathogens in green house grown tomatoes [Articol]
    (Editura USM, 2025) Deaghileva, Angela; Belousova, Galina; Cuznetova, Irina; Ignatova, Zoia; Muller, Karel
    Tomato production in protected environments such as greenhouses has become a popular trend in agriculture. The main advantage of greenhouse crop production over open field systems is the ability to control the environment, limiting the impact of abiotic and biotic factors. However, under greenhouse conditions tomatoes are still susceptible to certain soil-borne fungal diseases. Early detection of fungal pathogens is paramount for controlling the diseases and their spread. The aim of the study was to monitor fungal pathogens affecting tomato plants grown in protected conditions. Molecular diagnostics of pathogens Alternaria spp., Fusarium spp., Penicillium spp. and Aspergillus spp. was carried out during the vegetative season in various organs of tomato plants planting in greenhouse using nested-PCR assay. A. alternata was the predominant pathogen among all tested tomato genotypes. Fusarium and Aspergillus species were not detected in greenhouse tomato plantings during vegetative season.
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    Transcriptome profiling of tomato response to phytoplasma infection
    (CEP USM, 2024) Mitina, Irina; Grajdieru, Cristina; Bahsiev, Aighiuni; Tumanova, Lidia; Zamorzaeva, Irina; Mitin, Valentin; Juříček, Miloslav; Muller, Karel
    Transcriptome sequencing is a valuable tool, which allows the researchers to study the dynamics of gene expression and identify genes, which control economically important traits. In this work, genes involved in tomato response to ‘Candidatus Phytoplasma solani’, a bacterial pathogen which can cause severe economic losses, were studied by transcriptome sequencing. As a result, 143 genes were found to be differentially expressed in the infected tomato plants, compared to healthy plants, 81 were upregulated and 62 downregulated. This analysis can shed light on the mechanisms of phytoplasma induced host gene expression regulation and on tomato response to phytoplasma infection.