Browsing by Author "Grajdieru, Cristina"

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    DYNAMICS OF MAIZE PATHOGENS FROM FUSARIUM, ASPERGILLUS AND PENICILLIUM GENERA IN SOIL UNDER WEATHER CONDITIONS OF REPUBLIC OF MOLDOVA
    (CEP USM, 2023) Grajdieru, Cristina; Bilici, Elena
    The results of molecular expertise of phytosanitary state of upper layer of soil from experimental cornfields are presented. Several fungal pathogens from Fusarium, Aspergillus and Penicillium genera were identified using PCR assay. These pathogens were identified in soil samples during the three-year monitoring period and their respective quantities depended on weather conditions. Abiotic factors like air temperature and relative humidity have a significant impact on fungi’s propagation. Weather conditions of the year affected significantly accumulation of F. equiseti, F. graminearum, A. clavatus, P. expansum and P. chrysogenum in soil. A positive correlation between pathogens’quantities and values of relative air humidity was found.
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    EVALUAREA REZISTENȚEI A PLANTELOR DE PORUMB LA SPECIILE DE FUSARIUM PRIN METODA PCR
    (CEP USM, 2023) Tumanova, Lidia; Grajdieru, Cristina; Mitin, Valentin; Mitina, Irina
    Contaminarea câmpurilor de porumb cu speciile fungice de Fusarium duce la scăderea volumului și calității de roadă. În lucrarea dată, trei genotipuri de porumb la fazele de mătăsare, lapte-ceară și maturitate fiziologică au fost analizate pentru prezența infecțiilor mixte de F. verticillioides, F. oxysporum, F. avenaceum. Infecțiile mixte asimptomatice de F. oxysporum, F. verticillioides, F. avenaceum au fost identificate în toate mostrele de Ku123 și în unele mostre ale genotipului RF7. Genotipul MK01 a fost infectat doar cu F. oxysporum. Estimarea numărului de copii prin metoda PCR cu aplicarea diluțiilor seriale a demonstrat că genotipul Ku123 a fost cel mai susceptibil la infecție.
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    IMMUNOLOGIC EVALUATION OF MAIZE COLLECTION SAMPLES
    (CEP USM, 2023) Bilici, Elena; Grajdieru, Cristina
    Seven inbred maize lines from the active collection of Laboratory of plant genetic resources were tested for their susceptibility to fungal pathogens using visual evaluation of disease symptoms and molecular diagnostics. The highest infection rate was registered in MAN2492 and was 35%, therefore the respective genotype can be classified as susceptible. MAN2466 lacked symptoms of diseases and for MAN2488 minimum value of the respective parameter was registered (5%) – resistant genotype group. Quantitative analysis showed that the overall value of DNA sequences specific for the fungal genome was the lowest in the grain of MAN2488, MAN2491, MAN2470 genotypes.
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    Transcriptome profiling of tomato response to phytoplasma infection
    (CEP USM, 2024) Mitina, Irina; Grajdieru, Cristina; Bahsiev, Aighiuni; Tumanova, Lidia; Zamorzaeva, Irina; Mitin, Valentin; Juříček, Miloslav; Muller, Karel
    Transcriptome sequencing is a valuable tool, which allows the researchers to study the dynamics of gene expression and identify genes, which control economically important traits. In this work, genes involved in tomato response to ‘Candidatus Phytoplasma solani’, a bacterial pathogen which can cause severe economic losses, were studied by transcriptome sequencing. As a result, 143 genes were found to be differentially expressed in the infected tomato plants, compared to healthy plants, 81 were upregulated and 62 downregulated. This analysis can shed light on the mechanisms of phytoplasma induced host gene expression regulation and on tomato response to phytoplasma infection.