Browsing by Author "Chiselita, Oleg"
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Item THE EFFECTS OF SOME COMPOUNDS OF Mn(II) AND Zn(II) ON THE MULTIPLICATION OF WINE YEAST AND BIOSYNTHESIS OF CARBOHYDRATES IN THE BIOMASS(University of Oradea Publishing House, 2010) Usatîi, Agafia; Chiselita, Oleg; Rudic, Valeriu; Chiselita, Natalia; Molodoi, Elena; Efremova, Nadejda; Gulea, Aurelian; Turta, Constantin; Lozan, VasileThe aim of investigation was to establish the influence of sulphates, acetates and coordination compounds of Mn(II) and Zn(II) as stimulators of the multiplication and growth of wine yeasts strain Saccharomyces cerevisiae CNMN-Y-20 and inducers of carbohydrate biosynthesis in biomass with the following elaboration of new procedures for directed carbohydrate biosynthesis. The study has been revealed that compounds MnSO 4 •4H 2 O, [Mn 2 Ac(2PyTCH)-1,5-Bis(piridin)tiocarbohidrazid- dimanganese-acetate] and ZnLP-2 in optimal concentrations can be recommended as effective regulators of the yeast Saccharomyces cerevisiae CNMN-Y-20 multiplications, as well as compounds [Mn(Gly) 2 ]Cl 2, (CH 3 COO) 2 Zn and ZnLP-2 in established concentrations - of the carbohydrates biosynthesis in the biomass of studied wine yeast strain. Stimulatory properties of studied compounds of Mn 2+ and Zn 2+ on carbohydrate biosynthesis in biomass of Saccharomyces cerevisiae CNMN-Y-20 can be explained both by the action of metal and by the nature of the ligand. The obtained biomass of wine yeast strain with high content of carbohydrates can be utilized for the obtaining of new bioproducts with the following application in the field of medicine and cosmetology.Item VIABILITY AND ANTIMICROBIAL ACTIVITY OF STREPTOMYCES STRAINS FROM NCNM AFTER LYOPHILIZATION(CEP USM, 2016) Chiselita, Oleg; Burţeva, Svetlana; Bîrsa, Maxim; Bulimaga, Valentina; Vasiliciuc, AnastasiaThe article deals with the aspects related to lyophilization of streptomycetes strains, preserved in the National Collection of Nonpathogenic Microorganisms (NCNM). Was determined that lyophilization do not significantly modify the antimicrobial activity of streptomycetes. Maximum viability of strains of genus Streptomyces (83,2-90,2%) is ensured after lyophilization at initial titer by 9-11 log10UFC ml-1 in protective medium (gelatin 2,5% + glucose 7,5%) by rehydration with distillate water