Browsing by Author "Cauș, Maria"
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Item ACTIVITATEA CATALAZEI, PEROXIDAZEI ȘI PEROXIDĂRII LIPIDELOR ÎN RĂDĂCINILE PORUMBULUI SUB INFLUENȚA ȘOCULUI TERMIC ȘI A PREPARATLUI REGLALG(CEP USM, 2023) Cauș, Maria; Dascaliuc, A.; Borozan, P.In recent years, due to the climate changes, the growth and development of corn is affected by heat stress, especially at an early stage of ontogeny. Influence of biostimulant Reglalg, used for seed pretreatment of maize hybrids Por. 310 and Por. 374 before exposure to heat shock (HS) at 50°C for 30 min, on catalase (CAT), peroxidase (PO), and lipid peroxidation (LPO) of root seedlings was studied. Compared to the Reglalg variants, root growth of control seedlings was more strongly affected by HS, since the activity levels of CAT, PO and MDA content in the roots were higher. This demonstrates the beneficial effects of Reglalg in increasing maize tolerance to HS.Item Efectul stresului de temperatură negativă asupra mobilizării rezervelor din semințe pentru germinare și creșterea timpurie a plantulelor diferitor hibrizi de porumb(CEP USM, 2024) Cauș, MariaA fost studiat efectul stresului de temperatură negativă (STN) de -4°C cu durata de 16 ore aplicat semințelor a 14 hibrizi de porumb înainte de germinare asupra mobilizării și translocării substanțelor de rezervă din endospermul semințelor în germinare și creștere a plantulelor. S-a stabilit că STN a avut un impact semnificativ asupra ratei de masă a endospermului (RME) consumată în formarea unei unități de masă de rădăcină, epicotil și respirație, prin inhibarea mobilizării masei endospermului în procesele de germinare și creștere a plantulelor. În baza datelor obținute, în funcție de % RME utilizată în germinare și creștere au fost evidențiați hibrizi cu valorile RME: (I) ≈ 24%-27% ; (II) ≈ 15% - 17% și (III) ≈ 5%-8%) mai mică, față de valorile variantelor martor.Item EVALUAREA REZISTENȚEI DIFERITOR HIBRIZI DE PORUMB LA STRESUL DE TEMPERATURĂ NEGATIVĂ ÎN STADIU DE GERMINARE A SEMINȚELOR(CEP USM, 2024) Cauș, Maria; Borozan, PanteleimonThe main objective of the study was to determine the constitutive resistance of 14maize hybrids to negative temperature stress. To achieve the proposed objective, the seedsf the experimental variants were exposed to hypothermic stress, being incubated at negative temperature of -4°C for 16 hours in the climatic chamber RUMED TYPE 3401 (RFG) with the accuracy of maintaining the temperature ± 0.1ºC. At the same time, control seeds were incubated at a temperature of +4°C. Then control and experimental seeds were germinated at an optimal temperature of +26°C for 5 days, followed by determination of the final percentage of germinaton. Pretreatment of maize seeds with negative temperature stress made it possible to identify and evaluate the primary stress resistance of each hybrid. Depending on the degree of constitutive frost resistance of each hybrid, the distribution and classification of the studied hybrids into 3 groups was carried out, including hybrids with high, medium and low frost resistance.Item Thermostability of peroxidase in leaves of Buxus sempervirens L. under the influence of hyperthermic shock [Articol](CEP USM, 2025) Cauș, Maria; Platcovschii, NicolaiThe aim of this study was to investigate the thermal stability of total activity and peroxidase (PO) isoenzymes isolated from boxwood Buxus sempervirens (L.) leaves subjected to hyperthermic shock (HTS) at temperatures in the range of 53-90°C for 5 min. It was demonstrated that PO in leaves exposed to HTS of 53°C, 55°C and 57°C exhibits high resistance to these temperatures, the activity being at the level of the control. Although, the total activity and intensities of PO isoenzymes distributed in 4 zones in a vertical gradient (4-7%) of polyacrylamide gel (PAAG) gradually decreased with increasing HTS of temperatures above 58°C. However, treatment of leaves with HTS at 90°C for 5 min resulted in complete suppression of PO, the activity of which was not detected.