Browsing by Author "Buzu, Ion"

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INFLUENCE OF SOME ORGANIC COORDINATION COMPOUNDS CONTAINING COBALT AND BISMUTH ON DEVELOPMENT MORPHO-PRODUCTIVE CHARACTERS OF THE BEE FAMILIES
(CERES Publishing House, 2015-09-17) Cebotari, Valentina; Buzu, Ion; Toderaș, Ion; Gulea, Aurelian; Postolachi, Olga; Toderici, Valeriu; Gliga, Olesea
The aim of the research was to evaluate and test the influence of organic coordination compounds containing rare microelements, on the vital functions and on the development of the morpho-character of Apis mellifera bee colonies. Several experiments where conducted and was tested in feeding bees in spring (April 1 to 21) a period with poor harvest in nature, a nutritious blend of 50% sugar syrup supplement enriched with bioactive organic compound heteronuclear coordinative sulfate [tris-thiosemicarbazide cobalt (III)] [1, 2-diaminociclohexantetraacetat bismuth (III)] hexahydrate - [Co (tios) 3], [Bi (CDTA)] SO4 · 6H2O in aqueous solution with concentration of 1 mg% (hereinafter referred to as "compound + Co + Bi), which was mixed with sugar syrup in a ratio of 2: 100, and was administered directly into the food of bees and bee families where feed with the nutrient in amount of 100 ... 130 ml for every frame with bees, every 2 days for a period of three weeks. Bee colonies were formed into three lots, each lot formed from 16 families, of which: group I - control, which were fed only with sugar syrup 50%, group II - prototype, which where feed with sugar syrup by adding nutritional supplement enriched with patented "Apispir + Fe + It" (MD 477 Z 2012.09.30) and group III - experimental, which received sugar syrup enriched with supplement "compound + Co + Bi". The research results have shown that using energizing nutritional supplements enriched with biologically active substances of organic compounds coordination in bee feeding in times when there is a poor harvest in nature, helps stimulate vital functions of bee families and increase their productivity: the prolificity of queen - with 5.2 to 9.7% (P <0.001); the amount of capped brood - with 5.2 to 9.7% (P <0.001); family strenght- by 2.5 to 9.7% (P <0.1s P <0.001); disease resistance - by 2.4 to 5.0% (P <0.001); brood viability - with 1.2 to 2.2% (P <0,01s (P <0.001); the amount of accumulated bee bread in brood chamber- by 15.0 to 23.3% (P <0.001), an increased amount of wax combs - by 21.4 to 39.3% (P <0.001) and the amount of honey accumulated in the colony - with 13.9 to 25.4% (P <0.01 and P <0.001). The beneficial effect of feeding bees with biologically active nutritional supplements Apispir + Fe + + Co + Se and Bi compound denotes the fact that in the spring (March-April), a period poor in harvesting, in the area studied, in nature there is a shortage of biologically active substances, including rare microelements.
INFLUENŢA REMEDIILOR ORGANICE BIOACTIVE DE GENERAŢIE NOUĂ ASUPRA ACTIVITĂŢII VITALE A FAMILIILOR DE ALBINE APIS MELLIFERA
(2014) Toderaș, Ion; Rudic, Valeriu; Gulea, Aurelian; Cebotari, Valentina; Buzu, Ion
Scopul cercetărilor a fost testarea comparativă, în hrana albinelor, a unor suplimente nutritive obținute din biomasa cianobacteriei Spirulina platensis, cultivate în prezenţa unor compuşi organici coordinativi de generaţie nouă şi elaborarea, în baza acesteia, a unor noi suplimente nutritive şi procedee de hrănire a familiilor de albine în perioadele deficitare de cules în natură. Cercetările au fost efectuate pe 5 loturi analoage cu familii de albine, a câte 10 familii în fiecare lot, care au primit în hrană la începutul lunii aprilie, peste o zi, pe parcursul a 2 săptămâni, câte 100 ml de amestec nutritiv de sirop din zahăr cu apă (1:1), la fiecare interval de faguri populat cu albină. Pentru familiile de albine din loturile experimentale (II, III, IV şi V) siropul de zahăr a fost îmbogăţit cu diferite suplimente nutritive obţinute din extractul biomasei cianobacteriei Spirulina platensis cultivate în prezenţa unor compuşi organici coordinativi. Suplimentele nutritive au fost administrate în cantitate de 20 mg substanţă uscată la 1L de amestec. Familiile de albine din lotul II au primit în hrană suplimentul bioactiv Apispir, lotul III - Apispir Zn, lotul IV - Apispir Cr şi din lotul V - Apispir Fe Se. Rezultatele cercetătilor au demonstrat că hrănirea albinelor, primăvara devreme, în perioada deficitară de cules în natură, cu amestec nutritiv din sirop de zahăr îmbogăţit cu suplimente bioactive obţinute din extractul biomasei Spirulina platensis cultivate în prezenţa unor compuşi organici coordinativi a contribuit la creşterea semnificativă a prolificităţii mătcii – cu 607 – 1390 ouă/24 ore sau 32,6–74,4 % (td = 2,93 – 8,99; P < 0,01 - 0,001), cantităţii de puiet căpăcit - cu 73– 67 sute celule sau 32,6–74,6 % (td = 2,92–8,99; P < 0,01-0,001), puterii familiei - cu 0,40–0,56 kg sau 18,1–25,3 % (td = 3,54–4,95; P < 0,01-0,001), intensităţii de zbor a albinelor - cu 1,89–2,21 albine/minut sau 19,7–23,1 % (td = 2,67–3,32; P < 0,05-0,01), rezistenţei la boli - cu 10,8–14,9 puncte absolute sau 13,4–18,5 % (td = 7,66–9,76; P < 0,001), viabilităţii puietului - cu 11,5–14,4 puncte absolute sau 14,2–17,7 % (td = 10,7–11,8; P < 0,001), cantităţii de ceară crescute în cuib - cu 1,46 – 1,71 faguri crescute sau 41,0 – 48,0 % (td = 2,46–2,78; P < 0,01), cantităţii de păstură - cu 17,9–36,4 sute celule sau 52,5–206,7 % (td = 2,76–6,59; P < 0,05-0,001) şi cantităţii de miere acumulate în cuib - cu 1,44–2,71 kg sau 53,1–90,0 % (td = 3,45–7,4; P < 0,01-0,001).
NEW NUTRITIVE SUPPLEMENTS FOR FEEDING MELLIFERA BEES IN THE DEFICIT PERIODS OF COLLECTION IN NATURE
(2017) Toderaș, Ion; Cebotari, Valentina; Ungureanu, Laurenția; Buzu, Ion; Gheorghiță, Cristina; Floquet, Sebastien; Gulea, Aurelian; Rudic, Valeriu; Fuior, Arcadie
The purpose of this paper was to test the bees feeding of aquatic microalgae biomass like Scenedesmus quadricauda, Scenedesmus Apiculatus Hortob, Oocystis borgei Snow, and elaboration of feeding procedures of bee families during the deficit harvesting periods in nature, the end of winter and early spring (February - March). The research was carried out on Apis mellifera Carpatica bee families at the experimental apiary of the Institute of Zoology of the Academy of Sciences of Moldova. At the end of February, 5 similar batches of bees were formed, with 13-15 families in each batch. Each bee family was given once, 200g in a 7:3 ratio of mixture of sugar powder with honey, the consistency of a dense gelatinous paste in disk form, a disk at each bee frame. In batch I (witness), the bees received only the nutritive paste made of sugar powder + honey; In batch II the bees received nutrient paste enriched with the nutritional supplement known as Apispir in quantity of 2 ml of solution by 10% (200 mg active substance) per 1 kg of paste; In batches III, IV and V, the bees received the food nutritive paste, enriched with the biomass of aquatic microalgae, respectively: Scenedesmus quadricauda, Scenedesmus apiculatus and Oocystis borgei Snow, in quantity of 10 ml of suspension by 2.0% concentration (200 mg dry substance) to 1 kg of paste. At 100 days from the date of feeding the bees with nutritive paste (which coincided with the first collection), at each family in the experimental batches, individually, was evaluated: the queen prolificacy, the capped brood quantity, the strength of the family, the resistance to diseases, the viability of the brood, the quantity of wax increased on the combs, the quantity of pasture and honey accumulated in the nest. The results of the research have demonstrated that feeding bee families during deficit periods of harvesting in nature with a 7: 3 ratio sugar-powder + honey, nutritional mixture, enriched with biologically active supplements from Scenedesmus quadricauda, Scenedesmus apiculatus and Oocystis borgei Snow contributes to the increase, compared to the control batch: of the prolificacy of the queens by 7.8-10.3% (td = 3.5-5.2; P <0.01-0.001); the quantity of brood - by 7.7-9.3% (td = 3.4-4.7; P <0.01-0.001); the strength of the bee family - by 7.1-9.3% (td = 3.0-3.5, P <0.01); resistance of families to diseases - by 1.8-3.8% (td = 1.6-3.6; P <0.1-0.01); viability of brood - by 1.2-1.7% (td = 2.2-2.9; P <0.05-0.01); wax production increased by 13.3-36.7% (td = 2.9-6.4; P <0.01-0.001); the quantity of bee bread accumulated in the nest - by 15.527.6% (td = 3.4- 8.4, P <0.01-0.001); the quantity of honey accumulated in the nest at the first harvest - by 28.0-38.9% (td = 7.2-9.9, P <0.001). On the basis of the researches have elaboration, new procedures of feeding bee families during the deficit periods of harvesting in the nature and obtained patents of invention (MD 1061 Y 2016.08.31, MD 1079 Y 2016.08.31, MD 1079 Y 2016.10.31).