Browsing by Author "Buzan, Vladimir"

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    ADVANTAGES OF CRYOCONSERVATION OF SPERM IN REPRODUCTIVE BIOTECHNOLOGY
    (CEP USM, 2022-09-29) Roșca, Nicolae; Balan, Ion; Balacci, Sergiu; Buzan, Vladimir; Crețu, Roman; Moroz, Mihail; Osipciuc, Galina; Bacu, Gheorghe
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    DIRECTING THE GROWTH OF CARP LARVAE THROUGH THE APPLICATION OF THERMAL FACTOR
    (CEP USM, 2022-09-29) Balacci, Sergiu; Balan, Ion; Buzan, Vladimir
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    EVOLUȚIA TEHNICILOR DE EVALUARE ALE MATERIALULUI SEMINAL ÎN DETERMINAREA FERTILITĂȚII MASCULINE
    (CEP USM, 2022-11-10) Balan, Ion; Balacci, Sergiu; Roşca, Nicolae; Buzan, Vladimir; Furdui, Vlada; Mereuţă, Ion; Harea, Vasile; Hanțațuc, Alexei; Vîhrist, Ecaterina; Temciuc, Vlad
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    INFLUENȚA FACTORILOR INTRINSECI ȘI EXTRINSECI ASUPRA DEZVOLTĂRII CELULELOR SOMATICE ȘI GERMINALE
    (CEP USM, 2024) Balan, Ion; Gavojdian, Dinu; Roşca, Nicolae; Balacci, Serghei; Buzan, Vladimir; Furdui, Vlada; Osipciuc, Galina; Harea, Vasile
    Mammalian spermatogenesis is the most complex regulated process of cell development and represents a specific physiological process for the study of the peculiarities of the mechanism of spermatogenesis and for perceiving the basic evolution of all developmental processes, since it involves specific molecular processes of somatic and sex cells. Spermatogenesis generates spermatozoa from spermatogonal stem cells through several stages and comprises spermatocytogenesis, meiosis and spermiogenesis with the continuous unfolding of molecular events of mitosis, meiosis and epigenetic dynamic changes at the morphological and molecular level. Spermatogenesis establishes some dynamic changes by overlapping the functions of different types of cells and is maintained by different physiological factors impacting testicular morphofunction. The existing overlaps complicate the conclusive elucidation of the individual roles of different somatic and germ cells in the self-renewal, proliferation and apoptosis of spermatogonial stem cells and create difficulties in understanding the contribution of distinct signaling molecules during spermatogenesis. Several factors, compounds secreted by all somatic cells, including Sertoli cells, peritubular myoid cells and Leydig cells are involved in the regulation and development of spermatogenesis, but the mechanisms by which these factors interact in somatic and germ cells are largely unknown and a comprehensive study of these cells is needed.
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    INFLUENȚA MEDIULUI ZAHAROZĂ, DULCIT, ARGININĂ ȘI VITAMINA B6 PENTRU CONSERVAREA SPERMEI UMANE
    (CEP USM, 2024) Cazacov, Iulia; Balan, Ion; Roşca, Nicolae; Buzan, Vladimir; Mereuța, Ion; Bucarciuc, Melania
    Au fost studiați indicii fiziologici care caracterizează sperma sanogenă de om prin conservare cu folosirea argininei și vitaminei B6 în componența mediului de zaharoză și dulcit. În rezultatul cercetărilor, diluantul cu conținut de vitamina B6 1,6 mg și arginină 0,29 mg, pe bază de zaharoză, dulcit-glicerină-gălbenuș și apă distilată după congelare-decongelare a celulelor sexuale umane, posedă indici fiziologici care caracterizează sperma sanogenă procentual (mobilitatea 29,7 %, indicile absolut al supraviețuirii 93,6 % și longevitatea 28,3%) mai sporit comparativ cu lotul martor
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    INFLUIENȚA EXTRACTULUI DE POLIFENOLI DIN NUCILE VERZI ASUPRA METABOLISMULUI GLUTATIONULUI DIN SERIUL SANGUIN AL COCOȘILOR
    (CEP USM, 2024) Roșca, Nicolae; Balan, Ion; Buzan, Vladimir; Balacci, Sergiu; Furdui, Vlada; Mereută, Ion; Osipciuc, Galina; Crețu, Roman; Bacu, Gheorghii
    In this paper, the influence of polyphenol extract from green walnuts on glutathione metabo lism in the blood serum of breeding roosters was studied. To achieve this purpose, hydroalcoholic extract from green walnuts was used, with a total antioxidant activity of 548,37 mg gallic acid equivalent/100 gr. For the study were used two batches of roosters, of five animals in each batch, experimental and control. As a result of the research on the influence of metabolism, changes were obtained in the content of SH-groups, G-TP, G-S-T, as well as amino acids, which are part of the glutathione composition and which were studied in different biological fluids and repro ductive cells. The basic mechanism of the central role of thiol-mediated redox control in cellular metabolism is the ability of thiol groups to reversibly change their redox state with subsequent changes in the conformational, catalytic or regulatory functions of the protein.
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    Morfofuncționalitatea mitocondrială în evoluția spermatogenezei la mamifere
    (Editura USM, 2024) Balan, Ion; Surai, Peter; Marin, Paula Monica; Balacci, Sergiu; Roșca, Nicolae; Buzan, Vladimir; Petcu, Igor; Mereuță, Ion; Osipciuc, Galina; Popa, Valerian; Vîhrist, Ecaterina
    In spermatogenesis, mitochondria are particularly important for the development and maintenance of germ cells and the production of healthy gametes. Deficiencies in these processes lead to mitochondrial dysfunction and abnormal spermatogenesis. The classical roles of mitochondria in energy production, metabolism and apoptosis have been well defined, but they also have various other functions. Mitochondria are also involved in testosterone production, regulating proton secretion in the lumen to maintain an acidic state in the epididymis, and condensing sperm DNA. In the available literature on sperm mitochondria, it is increasingly recognized that the morphology and functional status of mitochondria has a significant impact on sperm characteristics. Although mitochondria are recognized as essential for sperm, their exact pathophysiological role and functioning are complex. Understanding and managing mitochondrial quality control could be a valuable approach for developing new strategies in the physiological evolution of spermatogenesis.
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    RELEVANȚA BIOLOGIEI REPRODUCERII ȘI CONSERVĂRII ÎN CONTINUITATEA BIODIVERSITĂȚII
    (CEP USM, 2024) Balan, Ion; Drugociu, Dan Gheorghe; Roşca, Nicolae; Balacci, Sergiu; Buzan, Vladimir; Furdui, Vlada; Harea, Vasile; Hanțațuc, Alexei; Moroz, Mihail; Turcanu, Parascovia; Olaru, Iulia
    Wild and domestic animal breeding programs, a vital insurance strategy to conserve the biodiversity of endangered species and to facilitate the field of research that offers solutions to many aspects of the biodiversity crisis. Reproduction is a particularly essential component of conservation, where the protection of life remains one of society’s highest priorities. When applying the biology of long-term or short-term preservation of preserved semen, it is necessary to take into account the specific aspects of cryobiology, or sperm dehydration, with the reco very of cellular and nuclear structures, being the major contributors, both to the preservation of biodiversity and How. to the development of reproductive sciences.Currently, advances in the preservation of reproductive cells and the creation of cryogenics of genetic resources offer decisive solutions in the maintenance of biodiversity, and their strategy depends on the amount and duration of storage of frozen semen for several generations to come.
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    THE ROLE AND IMPORTANCE OF REPRODUCTIVE BIOTECHNOLOGIES IN ANIMAL BIODIVERSITY
    (CEP USM, 2022-09-29) Balan, Ion; Roșca, Nicolae; Buzan, Vladimir; Balacci, Sergiu; Cazacova, Iulia; Mereuța, Ion; Crețu, Roman; Bacu, Gheorghe