Browsing by Author "Belousova, Galina"
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Item Identificarea moleculară a speciilor de fusarium în seminţe de susan (Sesamum indicum L.)(CEP USM, 2024-10-07) Belousova, Galina; Mogîlda, AnatoliiThe research focused on the identification of pathogenic fungi of the genus Fusarium in sesame seeds. To evaluate the spectrum of pathogens in seeds, molecular analysis based on nested-PCR was performed. Twelve samples of DNA extracted from sesame seeds obtained under field conditions were analyzed. The analysis revealed the presence of Fusarium spp. in 11 DNA samples tested. Discrimination analysis of 4 pathogen species was performed: F. culmorum, F. equiseti, F. nivali, F. avenaceum. The species F. culmorum and F. équiseti were identified in six samples. The pathogenic fungi F. nivali and F. avenaceum were not identified in the analyzed samples.Item Molecular diagnostics of some fungal pathogens in green house grown tomatoes [Articol](Editura USM, 2025) Deaghileva, Angela; Belousova, Galina; Cuznetova, Irina; Ignatova, Zoia; Muller, KarelTomato production in protected environments such as greenhouses has become a popular trend in agriculture. The main advantage of greenhouse crop production over open field systems is the ability to control the environment, limiting the impact of abiotic and biotic factors. However, under greenhouse conditions tomatoes are still susceptible to certain soil-borne fungal diseases. Early detection of fungal pathogens is paramount for controlling the diseases and their spread. The aim of the study was to monitor fungal pathogens affecting tomato plants grown in protected conditions. Molecular diagnostics of pathogens Alternaria spp., Fusarium spp., Penicillium spp. and Aspergillus spp. was carried out during the vegetative season in various organs of tomato plants planting in greenhouse using nested-PCR assay. A. alternata was the predominant pathogen among all tested tomato genotypes. Fusarium and Aspergillus species were not detected in greenhouse tomato plantings during vegetative season.